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Biomarkers and Progress Tracking: What Your Labs Can (and Can’t) Tell Us

Published on
June 2, 2025

In complex chronic illness—especially those involving infections, inflammation, and immune dysregulation—lab work can feel like a mix of science, strategy, and fortune-telling. Biomarkers can offer helpful clues about what's happening beneath the surface. They can suggest what might be driving symptoms, point us in the right treatment direction, and even help track progress over time.

But a word of caution: biomarkers are indicators—not oracles. They’re indirect, easily influenced by other factors, and not always reflective of how you feel. In our clinic, we use biomarkers to guide decisions, but your symptom response and overall progress always matter more.

Let’s look at a few of the biomarkers we often track, what they can (and can’t) tell us, and how we use them in context:

🔬 Eosinophil Cationic Protein (ECP)

What it tells us: ECP is released by eosinophils, immune cells involved in allergic and parasitic responses. When ECP is elevated (above ~10), it can suggest eosinophil-driven inflammation, which we often see with Candida overgrowth, Babesia, or environmental allergies.

Why it matters with Candida: Eosinophils can be triggered by yeast overgrowth in certain individuals—particularly when gut permeability, immune reactivity, or a Th2-dominant pattern is present.

How we use it: ECP helps confirm suspicion when symptoms and history suggest Candida or Babesia. Importantly, falling ECP levels during treatment can signal improvement, though this marker is not specific and can be influenced by other processes.

🧬 Vascular Endothelial Growth Factor (VEGF)

What it tells us: VEGF supports blood vessel formation and oxygen delivery. It’s often elevated in Bartonella infections, which inflame the vasculature and contribute to symptoms like pain, headaches, and neurological changes.

The twist: In chronic biotoxin illness (CIRS), VEGF can become suppressed due to long-standing immune dysfunction. So values that appear “normal” may still be clinically significant.

How we use it:

  • VEGF >40 (even within the normal lab range) may suggest Bartonella in the context of CIRS.

  • VEGF >115 is strongly suggestive of Bartonella involvement.

We’ve also found that declining VEGF levels can reflect treatment success—though, again, this marker is only meaningful when interpreted alongside symptom changes and other labs.

🌞 1,25:25 Vitamin D Ratio

What it tells us: This ratio compares the active form of vitamin D (1,25-dihydroxy) with the storage form (25-hydroxy). A high ratio—typically greater than 2.0—suggests intracellular inflammation, which often occurs in the presence of infections like Borrelia (Lyme) or Bartonella.

Why it matters: Elevated 1,25 levels are a sign that the immune system is still in a heightened, dysregulated state. It may reflect ongoing immune activation even when other labs look normal—and can be falsely elevated if someone is taking high doses of vitamin D.

How we use it:
A high 1,25:25 ratio helps confirm that intracellular inflammation is still active, and often points us toward the need for intracellular antimicrobial treatment—whether prescription antibiotics or targeted herbal protocols. It's not a standalone marker, but when combined with symptoms and other labs, it helps guide timing and intensity of treatment.

🔥 Interleukin-6 (IL-6)

What it tells us: IL-6 is a pro-inflammatory cytokine, meaning it’s part of the signaling network that activates and escalates inflammation. High IL-6 tells us the immune system is still “on alert.”

How we use it:
IL-6 rises in response to chronic infections, mold toxins, trauma, or stress. Elevated levels help confirm that inflammation is still active and may guide us to reassess hidden triggers.

🧨 TNF-alpha (Tumor Necrosis Factor-alpha)

What it tells us: TNF-alpha is another key inflammatory cytokine, deeply involved in immune surveillance and response to infection. While IL-6 may signal general inflammation, TNF-alpha is more directly associated with persistent infection—especially intracellular or tissue-based microbes.

How we use it:
When used alongside IL-6, TNF-alpha gives us a clearer picture of whether infection is still active. Here's how we interpret the combo:

🔍 IL-6 + TNF-α Interpretation Guide: Clues to Infection Activity

Inflammation likely under control; symptoms and other markers guide next steps

These cytokines are not diagnostic on their own, but they can be powerful when used together—especially in complex or relapsing cases where infections may hide in tissues or biofilms.

💉 High-Sensitivity CRP (hsCRP)

What it tells us: hsCRP is a general inflammation marker, often elevated with Candida, blood sugar issues, or other inflammatory triggers.
How we use it:
It’s a reliable way to track systemic inflammation. Improvement in hsCRP during treatment typically reflects reduced inflammatory burden.

🔒 Ferritin

What it tells us: Ferritin stores iron—but it's also an acute-phase reactant, meaning it rises with inflammation.
How we use it:
Ferritin values >150 in women and >200 in men may reflect inflammation (not just iron overload). This is especially useful in tracking chronic inflammation, particularly in male patients.

📊 Biomarker Quick Guide: What We’re Looking For and Why It Matters

IL-6 TNF-α Interpretation
High High Strongly suggests active infection or persistent immune stimulation
High Normal May indicate resolving infection or immune activation without active pathogen
Biomarker What It May Indicate Concerning Values How We Use It
ECP Candida, Babesia, eosinophilic inflammation >10 Falling levels suggest improvement; context-dependent
VEGF Bartonella, vascular inflammation >40 may suggest; >115 strongly suggestive Helpful for Bartonella tracking; suppressed in CIRS
1,25:25 Vitamin D Ratio Intracellular inflammation (Borrelia, Bartonella) >2.0 Suggests immune activation; impacted by vitamin D intake
IL-6 Cytokine-driven inflammation Above reference range Signals ongoing immune activation
TNF-α Persistent infection Above reference range Useful with IL-6 to track infection vs immune activation
hsCRP Systemic inflammation (Candida, glucose issues) >1.0 (mild), >3.0 (high) Responsive to antifungals, detox, glucose balance
Ferritin Inflammation (not just iron status) >150 (women), >200 (men) Tracks chronic inflammation, especially in men

🧭 Final Thoughts: Symptoms > Lab Values

We love biomarkers because they give us clues. But we never forget they’re just one piece of the puzzle. Some patients feel significantly better long before their labs shift, while others show abnormal labs with few symptoms.

So, while we use these markers to guide and adjust treatment, we always prioritize how you're actually feeling. Healing is never just about fixing numbers—it's about restoring function, stability, and resilience in your body.

Your labs can inform your journey—but your body is still the compass.

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